Photo credit: Arne Aronsen

The genome of Mycena olivaceomarginata was sequenced as part of the Mycenaceae sequencing project and the overarching JGI 1000 Fungal Genomes project “Deep Sequencing of Ecologically-relevant Dikarya“ (CSP 1974).This project will examine members of the Mycena genus to evaluate the genomic basis of their different nutritional modes.

The brownedge bonnet, Mycena olivaceomarginata

Mycena olivaceomarginata is a member of the Rubromarginatae section (Maas Geesteranus 1988, Aronsen and Læssøe 2016). It has been reported frequently from Central and Northern Europe, and on a few occasions from N. America Asia and New Zealand, but appears not to be very common. It grows predominantly in grass and moss in open areas. It has a translucent-striated pileus, and is usually brownish-olivaceaously coloured on pileus, lamellae and stipe, but can be very variable. It always has the brownish coloured lamellar edge (cheilocystidia) characteristic of the section, and it is usually darker than its yellower sister species, M. citrinomarginata, which is also most often found in wooded habitats.

The collection from which the culture was grown was collected the 11th of August 2015 close to Longyearbyen at Svalbard, Arctic Norway. Mycena sequences have been found inside several living plant roots, especially in the Arctic (Botnen et al. 2014, Lorberau et al. 2017), and some of these sequences are closely similar to those of M. olivaceomarginata. This is why we included this Arctic M. olivaceomarginata specimen in the sequencing of Mycena.

This genome was derived from dikaryotic (diploid) pure culture on MEA agar with ampicilin and benomyl and should be free of xenobiotic contaminations. Researchers who wish to use data from unpublished Mycena genomes for publication are respectfully required to contact the PI and JGI to avoid potential conflicts on data use and coordinate other publications with the Mycena master paper(s).

References

Aronsen, A.; Læssøe, T.: The genus Mycena s.l. in The Fungi of Northern Europe, vol. 5. Copenhagen, 373 p (2016).

Botnen, S., Vik, U., Carlsen, T., Eidesen, P. B., Davey, M. L., & Kauserud, H. (2014). Low host specificity of root‐associated fungi at an Arctic site. Molecular ecology, 23(4), 975-985.

Maas Geesteranus, R. A. (1988): Conspectus of the Mycenas of the Northern Hemisphere. Proc. Kon. Ned. Akad. v. Wetensch. (Ser. C).

Lorberau, K. E., Botnen, S. S., Mundra, S., Aas, A. B., Rozema, J., Eidesen, P. B., & Kauserud, H. (2017). Does warming by open-top chambers induce change in the root-associated fungal community of the arctic dwarf shrub Cassiope tetragona (Ericaceae)?. Mycorrhiza, 27(5), 513-524.