Status
[November 2018] The Conocybe apala SZMC-NL-8967 genome was sequenced
with PacBio, assembled with Falcon (v1.8.8), and annotated with the
JGI Annotation Pipeline. Mitochondrial genome was assembled
separately and is available in the Download section.
The genome is likely a polymorphic dikaryon, and this is reflected
in an assembly and annotation with significant separation of
alleles. Many of the scaffolds are very similar to larger scaffolds
and are predicted to constitute an alternate or secondary
haplotype. To represent these primary and secondary haplotypes in
the Portal, we have created 'primary alleles' and 'secondary
alleles' gene model tracks, comprising the models found on each
haplotype. The goal of the GeneCatalog (GC) is to produce a
non-redundant set of models which captures the full functional
repertiore of the genome, and so the few secondary alleles that are
unique were included in the GC, while all others were not.
Genome Assembly | |
Genome Assembly size (Mbp) | 64.08 |
Sequencing read coverage depth | 190.11x |
# of contigs | 2441 |
# of scaffolds | 2441 |
# of scaffolds >= 2Kbp | 2187 |
Scaffold N50 | 227 |
Scaffold L50 (Mbp) | 0.06 |
# of gaps | 0 |
% of scaffold length in gaps | 0.0% |
Three largest Scaffolds (Mbp) | 0.95, 0.70, 0.66 |
ESTs | Data set | # sequences total | # mapped to genome | % mapped to genome |
EstClusters | ESTclusters | 139754 | 91516 | 65.5% |
Ests | est.fasta | 158815777 | 142374791 | 89.6% |
- Please note: Using BLAST, unmapped RNA reads and contigs were found to hit plant (Selaginella moellendorffii), as well as some Agaricomycetes (target organism, Chondrostereum purpureum, Rhizoctonia sp), suggesting that the RNA library was contaminated.
Gene Models | FilteredModels2 | |
length (bp) of: | average | median |
gene | 1783 | 1535 |
transcript | 1427 | 1229 |
exon | 240 | 148 |
intron | 74 | 60 |
description: | ||
protein length (aa) | 421 | 351 |
exons per gene | 5.95 | 5 |
# of gene models | 17284 |
Collaborators
- László Nagy, Hungarian Academy of Sciences, Hungary
- Joseph Spatafora, Oregon State University, OR, USA
Links
- 1000 Fungal Genomes Project
- JGI PhyloGroup Portals: Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricales