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Home • Kluyveromyces marxianus NRRL Y-1190 v1.0
Kluyveromyces marxianus colonies on lactose selective agar. Image generated by <a
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Kluyveromyces marxianus colonies on lactose selective agar. Image generated by Ude and available under the Creative Commons SA 3.0 License.

Kluyveromyces marxianus strains are thermotolerant, can metabolize a wide range of sugars, and are fast growing, thus they have significant biotechnological potential (Gombert, 2016). K. marxianus is frequently isolated from fermented beverages, and several studies have shown it to have a major role in flavor profiles (Morrissey, 2015). Thus, K. marxianus is an attractive platform host for biosynthesis of flavors and fragrances, in particular the important precursor ethyl acetate (Löser, 2014). A number of genome engineering tools have been developed for K. marxianus, including a CRISPR/Cas9 system (Nurcholis, 2020). Recently, the transformation competence of several K. marxianus isolates was evaluated (Cernak, 2018). K. marxianus NRRL Y-1190 (ATCC 8635, DSM 5421), had the highest transformation efficiency among the native strains tested. Thus, this strain is a promising host for future metabolic engineering and synthetic biology studies.

The Kluyveromyces marxianus NRRL Y-1190 genome was not sequenced by the JGI. The assembly was provided by Eric Young and it was annotated using the JGI Fungal Annotation Pipeline.

This strain was obtained from the ATCC, and was originally isolated from yogurt in West Germany.

References:

Gombert, A. K. et al. Kluyveromyces marxianus as a host for heterologous protein synthesis. Appl. Microbiol. Biotechnol. 100, 6193–6208, (2016).

Morrissey,  J. P. et al. Cell factory applications of the yeast Kluyveromyces marxianus for the biotechnological production of natural flavour and fragrance molecules. Yeast 32, 3–16, (2015).

Löser, C. et al. Perspectives for the biotechnological production of ethyl acetate by yeasts. Appl. Microbiol. Biotechnol. 98, 5397–5415, (2014).

Nurcholis,  M. et al. Integration of comprehensive data and biotechnological tools  for industrial applications of Kluyveromyces marxianus. Appl. Microbiol. Biotechnol. 104, 475–488, (2020).

Cernak, P. et al. Engineering Kluyveromyces marxianus as a robust synthetic biology platform host. mBio 9, (2018).