Status
(May 2016) The Lactarius quietus S23C genome was sequenced with PacBio, assembled with Falcon, and annotated with the JGI Annotation Pipeline.
Lactarius quietus S23C is likely a polymorphic dikaryon, and this is reflected in an assembly and annotation with significant separation of alleles. 1404 of the 2812 scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertiore of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.
Genome Assembly | |
Genome Assembly size (Mbp) | 115.90 |
Sequencing read coverage depth | 97x |
# of contigs | 2812 |
# of scaffolds | 2812 |
# of scaffolds >= 2Kbp | 2746 |
Scaffold N50 | 325 |
Scaffold L50 (Mbp) | 0.09 |
# of gaps | 0 |
% of scaffold length in gaps | 0.0% |
Three largest Scaffolds (Mbp) | 1.08, 0.90, 0.81 |
ESTs | Data set | # sequences total | # mapped to genome | % mapped to genome |
EstClusters | ESTclusters | 56406 | 52661 | 93.4% |
Ests | est.fasta | 180107308 | 177774464 | 98.7% |
Ests | NCBI_ESTs | 453 | 203 | 44.8% |
Gene Models | FilteredModels1 | |
length (bp) of: | average | median |
gene | 1556 | 1268 |
transcript | 1233 | 1001 |
exon | 224 | 146 |
intron | 73 | 57 |
description: | ||
protein length (aa) | 358 | 272 |
exons per gene | 5.52 | 4 |
# of gene models | 18943 |
Collaborators
- Francis Martin at MycorWeb
- Annegret Kohler at MycorWeb
Genome Reference(s)
Miyauchi S, Kiss E, Kuo A, Drula E, Kohler A, Sánchez-GarcÃa M, Morin E, Andreopoulos B, Barry KW, Bonito G, Buée M, Carver A, Chen C, Cichocki N, Clum A, Culley D, Crous PW, Fauchery L, Girlanda M, Hayes RD, Kéri Z, LaButti K, Lipzen A, Lombard V, Magnuson J, Maillard F, Murat C, Nolan M, Ohm RA, Pangilinan J, Pereira MF, Perotto S, Peter M, Pfister S, Riley R, Sitrit Y, Stielow JB, SzöllÅ‘si G, ŽifÄáková L, Å tursová M, Spatafora JW, Tedersoo L, Vaario LM, Yamada A, Yan M, Wang P, Xu J, Bruns T, Baldrian P, Vilgalys R, Dunand C, Henrissat B, Grigoriev IV, Hibbett D, Nagy LG, Martin FM
Large-scale genome sequencing of mycorrhizal fungi provides insights into the early evolution of symbiotic traits.
Nat Commun. 2020 Oct 12;11(1):5125. doi: 10.1038/s41467-020-18795-w