Status
[February 2020] The Phakopsora pachyrhizi K8108
v2.0 genome was sequenced with PacBio, assembled with MECAT,
and polished with arrow at Syngenta and annotated with the JGI
Annotation Pipeline. An alternative gene prediction set, determined
by Syngenta is also available .
Phakopsora pachyrhizi is likely a polymorphic dikaryon,
and this is reflected in an assembly and annotation with
significant separation of alleles. Many of the smaller scaffolds
are very similar to larger scaffolds and are predicted to
constitute an alternate or secondary haplotype. To represent these
primary and secondary haplotypes in the Portal, we have created
'primary alleles' and 'secondary alleles' gene model tracks,
comprising the models found on each haplotype. The goal of the
GeneCatalog (GC) is to produce a non-redundant set of models which
captures the full functional repertoire of the genome, and so the
few secondary alleles that are unique were included in the GC,
while all others were not.
Genome Assembly | |
Genome Assembly size (Mbp) | 1082.91 |
Sequencing read coverage depth | 64x |
# of contigs | 6507 |
# of scaffolds | 6505 |
# of scaffolds >= 2Kbp | 6505 |
Scaffold N50 | 900 |
Scaffold L50 (Mbp) | 0.28 |
# of gaps | 2 |
% of scaffold length in gaps | 0.0% |
Three largest Scaffolds (Mbp) | 3.03, 2.54, 2.54 |
ESTs | Data set | # sequences total | # mapped to genome | % mapped to genome |
EstClusters | ESTclusters | 244030 | 25609 | 10.5% |
Ests | est.fasta | 449553900 | 168432797 | 37.5% |
Gene Models | FilteredModels2 | |
length (bp) of: | average | median |
gene | 1637 | 1340 |
transcript | 1151 | 836 |
exon | 249 | 144 |
intron | 136 | 95 |
description: | ||
protein length (aa) | 337 | 235 |
exons per gene | 4.62 | 4 |
# of gene models | 18216 |
Collaborators
Project management:
- Gabriel Scalliet (Syngenta, Switzerland)
- Ulrich Schaffrath (RWTH Aachen University, Germany)
- Uwe Conrath (RWTH Aachen University, Germany)
- Qingli Liu (Syngenta, USA)
Bioinformatics:
- Andrew Farmer (Syngenta / NCGR, USA)
- Connor Cameron (Syngenta /NCGR, USA)
- Stephanie Widdison (Syngenta / General Bioinformatics, UK)
- Eric Ganko (Syngenta, USA)
Sampling:
- Marco Löhrer (RWTH Aachen University, Germany)
- Stefan Bohnert (RWTH Aachen University, Germany)
- Sabine Kind (RWTH Aachen University, Germany)
- Dirk Balmer (Syngenta, Switzerland)
Genome Reference(s)
Gupta YK, Marcelino-Guimarães FC, Lorrain C, Farmer A, Haridas S, Ferreira EGC, Lopes-Caitar VS, Oliveira LS, Morin E, Widdison S, Cameron C, Inoue Y, Thor K, Robinson K, Drula E, Henrissat B, LaButti K, Bini AMR, Paget E, Singan V, Daum C, Dorme C, van Hoek M, Janssen A, Chandat L, Tarriotte Y, Richardson J, Melo BDVA, Wittenberg AHJ, Schneiders H, Peyrard S, Zanardo LG, Holtman VC, Coulombier-Chauvel F, Link TI, Balmer D, Müller AN, Kind S, Bohnert S, Wirtz L, Chen C, Yan M, Ng V, Gautier P, Meyer MC, Voegele RT, Liu Q, Grigoriev IV, Conrath U, Brommonschenkel SH, Loehrer M, Schaffrath U, Sirven C, Scalliet G, Duplessis S, van Esse HP
Major proliferation of transposable elements shaped the genome of the soybean rust pathogen Phakopsora pachyrhizi.
Nat Commun. 2023 Apr 1;14(1):1835. doi: 10.1038/s41467-023-37551-4
Links
- JGI PhyloGroup Portals: Fungi Dikarya Basidiomycota Pucciniomycotina Pucciniales Phakopsora
- JGI EcoGroup Portals: Plant Pathogens