Status
[October 2022] The Sarocladium kiliense UNK genome is one of two metagenome-extracted genomes from a set of contaminated samples. Sequenced by Illumina, binned DNA reads for Sarocladium kiliense UNK and Aspergillus bicolor CBS425.77 were assembled separately by SPAdes, and a shared transcriptome library was assembled by Trinity. Subsequently, the JGI Annotation Pipeline produced structural and functional annotations.
| Genome Assembly | |
| Genome Assembly size (Mbp) | 36.43 |
| Sequencing read coverage depth | 96.45x |
| # of contigs | 161 |
| # of scaffolds | 146 |
| # of scaffolds >= 2Kbp | 127 |
| Scaffold N50 | 10 |
| Scaffold L50 (Mbp) | 1.24 |
| # of gaps | 15 |
| % of scaffold length in gaps | 0.0% |
| Three largest Scaffolds (Mbp) | 3.01, 2.74, 2.49 |
Note: this transcriptome library was determined to be a sample
heavily contaminated with Aspergillus bicolor CBS425.77.
Therefore, mapping to Sarocladium kiliense UNK is low
compared to typical axenic RNAseq libraries.
| ESTs | Data set | # sequences total | # mapped to genome | % mapped to genome |
| EstClusters | ESTclusters | 51409 | 20426 | 39.7% |
| Ests | est.fasta | 15305578 | 6169767 | 40.3% |
| Gene Models | FilteredModels1 | |
| length (bp) of: | average | median |
| gene | 1818 | 1566 |
| transcript | 1632 | 1426 |
| exon | 538 | 321 |
| intron | 94 | 62 |
| description: | ||
| protein length (aa) | 502 | 431 |
| exons per gene | 3.03 | 3 |
| # of gene models | 12341 |
Collaborators
- Scott Baker at DOE Environmental Molecular Sciences Laboratory, Richland, WA, USA
- Mikael Andersen at Novozymes A/S, Denmark
Links
- JGI PhyloGroup Portals: Fungi Dikarya Ascomycota Pezizomycotina Sordariomycetes Hypocreomycetidae Hypocreales
Funding
The work conducted by the U.S. Department of Energy Joint Genome
Institute, a DOE Office of Science User Facility, is supported by
the Office of Science of the U.S. Department of Energy under
Contract No. DE-AC02-05CH11231.
