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Home • Metschnikowia reukaufii MR1 v1.1
Metschnikowia reukaufii budding cells and chlamydospores
Metschnikowia reukaufii budding cells and chlamydospores. Bar = 10 µm. M. reukaufii cells grown in YM media. Image credit: Dhami MK

The genome of Metschnikowia reukaufii MR1 was sequenced and assembled by Manpreet Dhami and Tadashi Fukami at Stanford University. The JGI Fungal Annotation pipeline was used to predict genes and provide functional annotation.

Metschnikowia reukaufii (Saccharomycetales: Metschnikowiaceae) is a diploid ascomycete yeast with a genome size of ~19 Mb(1). M. reukaufii is a ubiquitous yeast that lives in the floral nectar of many plant species (2–9). M. reukaufii exerts particularly strong priority effects against other species of nectar-inhabiting yeast and bacteria (8, 10, 11), potentially due to competition over limited N in floral nectar (1).
The sequenced strains of M. reukaufii were isolated from the nectar of Diplacus aurantiacus (Sticky Monkey Flower) flowers harvested from around the Bay Area of California. The methods for strain collection and sequenced genomes of strains studied here were published previously (12).
Researchers who wish to publish analyses using data from unpublished CSP genomes and/or transcriptomes are respectfully required to contact the PI and JGI to avoid potential conflicts on data use and coordinate other publications with the CSP master paper(s).

References:

  1. M. K. Dhami, T. Hartwig, T. Fukami, Genetic basis of priority effects: insights from nectar yeast. Proc. R. Soc. B. 283, 20161455 (2016).
  2. D. Eisikowitch, M. A. Lachance, P. G. Kevan, S. Willis, D. L. Collins-Thompson, The effect of the natural assemblage of microorganisms and selected strains of the yeast Metschnikowia reukaufii in controlling the germination of pollen of the common milkweed Asclepias syriaca. Can. J. Bot. 68, 1163–1165 (1990).
  3. C. M. Herrera, Population growth of the floricolous yeast Metschnikowia reukaufii: effects of nectar host, yeast genotype, and host × genotype interaction. FEMS Microbiol Ecol. 88, 250–257 (2014).
  4. C. M. Herrera, C. de Vega, A. Canto, M. I. Pozo, Yeasts in floral nectar: a quantitative survey. Ann Bot. 103, 1415–1423 (2009).
  5. C. M. Herrera, M. I. Pozo, P. Bazaga, Nonrandom genotype distribution among floral hosts contributes to local and regional genetic diversity in the nectar–living yeast Metschnikowia reukaufii. FEMS Microbiol Ecol. 87, 568–575 (2014).
  6. M. I. Pozo, M.-A. Lachance, C. M. Herrera, Nectar yeasts of two southern Spanish plants: the roles of immigration and physiological traits in community assembly. FEMS Microbiol Ecol. 80, 281–293 (2012).
  7. A. Canto, C. M. Herrera, R. Rodriguez, Nectar-living yeasts of a tropical host plant community: diversity and effects on community-wide floral nectar traits. PeerJ. 5, e3517 (2017).
  8. K. G. Peay, M. Belisle, T. Fukami, Phylogenetic relatedness predicts priority effects in nectar yeast communities. Proceedings of the Royal Society B: Biological Sciences. 279, 749–758 (2012).
  9. K. G. Peay, M. Belisle, T. Fukami, Phylogenetic relatedness predicts priority effects in nectar yeast communities. Proceedings of the Royal Society of London B: Biological Sciences, 749–758 (2012).
  10. R. L. Vannette, T. Fukami, Historical contingency in species interactions: towards niche-based predictions. Ecol Lett. 17, 115–124 (2014).
  11. C. M. Tucker, T. Fukami, Environmental variability counteracts priority effects to facilitate species coexistence: evidence from nectar microbes. Proceedings of the Royal Society B: Biological Sciences (2014).
  12. M. K. Dhami, T. Hartwig, A. D. Letten, M. Banf, T. Fukami, Genomic diversity of a nectar yeast clusters into metabolically, but not geographically, distinct lineages. Molecular Ecology (2018), doi:10.1111/mec.14535.